prediction using pure standards is expected to be biased whenever the slope of the calibration is affected by the presence of sample matrix. moreover, in the presence of unknown spectral interferents, first-order algorithms like partial least squares cannot be used. in this study, a method for determination of carvedilol (car) in tablet and urine samples is proposed by excitation-emission fluor...

second order advantage of excitation-emission fluorescence matrix was applied for the simultaneous determination of ibuprofen and caffeine. the proposed method is based on the measurement of the native fluorescence and recording emission spectra of ibuprofen and caffeine in different excitation wavelengths. the mixture of these compounds was resolved by multivariate curve resolution coupled wit...